Faecal samples, carcass swabs, knife swabs and personnel hand swabs from transport medium was streaked on to Campylobacter blood free selective agar base called modified Cefoperazone Charcoal Deoxycholate Agar (mCCDA from Oxoid Ltd.) that supplemented with SR155H supplement, and non-blood free Campylobacter selective agar base with campylobacter supplement (Blaserwanger: from HIMEDIA). Please enter a term before submitting your search. isolated from meats, animal, and human feces in Northern of Morocco. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. Research Highlights The acknowledgement part is corrected in the revised manuscript. We reformulated the tittle as Isolation, identification and antibiotic resistance profile of thermophilic Campylobacter species from bovine, knives and personnel at Jimma Town abattoir, Ethiopia. 2022 Elsevier Ltd. All rights reserved. Collection Contents 20 results [ALL] A B C D E F The overall prevalence of thermophilic Campylobacterspecies was 5.6% (38/684). The focus for inviting donor investment is likely to remain on the three diseases that have been the hallmark of its success. Public Health England. Campylobacters also harbor AMR genes with the capability of horizontal transfer between pathogenic and commensal microorganisms, which could lead possibly to the emergence of multi-drug resistant (MDR) microorganisms [12]. The front cover artwork from the Viewpoint by Jin et. British Journal of General Practice is an editorially-independent . Atlanta, GA: U.S. Department of Health and Human Services, CDC; 2019. Open Highlights present themed summaries of content from across the PLOS journals and other Open Access sources. On the other hand, antimicrobial resistance (AMR) is recognized as a One Health challenge because of the rapid emergence and dissemination of resistant microbial genes among humans, animals and the environment. "Upon re-submitting your revised manuscript, please upload your studys minimal underlying data set as either Supporting Information files or to a stable, public repository and include the relevant URLs, DOIs, or accession numbers within your revised cover letter. Antibiotic Resistance Threats in the United States, 2019. A cross-sectional study with systematic sampling method was conducted from October 2019 to September 2020 for the isolation, identification and antimicrobial susceptibility pattern of thermophilic Campylobacter species. These genetic mutations can arise from the adoption of a plasmid encoding a resistance gene or by mutation to the bacterial chromosome itself. 11 April 2022. Previous study conducted in Jimma Zone urban and rural farm animals also documented comparable prevalence (12.7%) of Campylobacter species from cattle fecal samples [15]. The predominant thermophilic Campylobacter species isolate in the current study was C. jejuni followed by C. coli and C. lari, respectively. Epub 2010 Feb 10. The clinical pipeline for antibiotics is too small to tackle the increasing emergence and spread of AMR. | After two to three days incubation under micro-aerobic condition, mCCDA plates were assessed visually for presence of Campylobacter species colonies. These cookies allow us to count visits and traffic sources so we can measure and improve the performance of our site. . Authors Niels Hiby 1 . Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. Antimicrobial Resistance (AMR) occurs when microorganisms develop resistance to antimicrobial medications such as antivirals and antibiotics. b. If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. You can review and change the way we collect information below. The susceptibility of Campylobacter to available drugs is tending decreased and continuous assessment of AMR at different sites and regions is essential [13, 14]. Neogi SB, Islam MM, Islam SKS, Akhter AHMT, Sikder MMH, Yamasaki S, et al. You are using a browser version with limited support for CSS. Although poultry is considered as the main source of infection for human, cattle also play substantial role for transmission of the disease in Ethiopia, particularly where consumption of raw beef is a culture [28]. 2010 Apr;35(4):322-32. doi: 10.1016/j.ijantimicag.2009.12.011. As you can see on the reviewers comments, it was some controversy with this manuscript. Similarly, Campylobacter species were isolated from hand and knife swabs, and it could be due to cross contamination due to negligence of hand and knife washing in between cattle slaughtering. Here the authors report a method for phenotypic AST at the single cell level, using a microfluidic chip that allows for subsequent genotyping with in situ FISH; they apply this to a mixed sample of 7 species and 4 antibiotics. Meera Tandan, Poshan Thapa, Preeti Maharjan, Buna Bhandari Open Access. Here, we comment on our collaborative efforts at establishing single-cell capacities in sub-Saharan Africa and the challenges encountered in Malis endemic setting. Asmai R, Karraouan B, Es-Soucratti K, En-Nassiri H, Bouchrif B, Karib H, et al. Please see our guidelines for more information on what we consider unacceptable restrictions to publicly sharing data: http://journals.plos.org/plosone/s/data-availability#loc-unacceptable-data-access-restrictions. Antimicrobial resistance is the ability of microorganisms such as bacteria, fungi or protozoans to grow despite exposure to antimicrobial substances designed to inhibit their growth. Other readers may not be benefited by the data. The finding was in agreement with the result documented in Gambella, Ethiopia [16]. The research finding will benefit the local and national government to take measures in Jimma abattoir in particular and abattoirs in the country in general. The results showed that Salmonella enterica serovars Enteritidis and Typhimurium were the common serotypes (56%; 25%). The aim of this study was to characterize the prevalence of pathogenic Salmonella serovars, antimicrobial susceptibility, and antimicrobial resistance genes in 112 Salmonella isolates recovered from raw poultry meat products. If you have any billing related questions, please contact our Author Billing department directly at gro.solp@gnillibrohtua. Antimicrobial resistance constitutes a global burden and is one of the major threats to public health. al. Antimicrobial resistance of Campylobacter isolates from small scale and backyard chicken in Kenya. The British Society for Antimicrobial Chemotherapy is an inter-professional organisation with over 40 years of experience and achievement in antibiotic education, research and leadership. Interpretive criteria [24] for Campylobacter susceptibility testing recommended by the Clinical and Laboratory Standards Institute and zone diameter breakpoints for Enterobacteriaceae were used [25]. PLOS defines a study's minimal data set as the underlying data used to reach the conclusions drawn in the manuscript and any additional data required to replicate the reported study findings in their entirety. and JavaScript. Study conducted in Mekele, Northern Ethiopia also reported higher prevalence (11.9%) of Campylobacter species isolates from cattle meat [17]. Has the statistical analysis been performed appropriately and rigorously? It has proven itself a highly effective instrument for protecting susceptible populations from preventable and treatable diseases. 01 April 2022. UK Standards for Microbiology Investigations. Turbidity of the broth culture was measured with turbido-meter with in absorbance reading range of 0.08 to 0.1 at 625nm (equivalent to 0.5 McFarland turbidity standards). The PLOS ONE style templates can be found at, https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and, https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf. The method uses previously generated chemical-genetic datasets and as a proof-of-concept, the researchers identified new synergistic combinations, including one with the classic antiviral, AZT. The field covered is broad and includes the physical, pharmacological, and in vitro properties of antiviral agents, antibacterial agents, antifungal agents, antiparasitic agents, etc., as well as . As bacteria that cause infection adapt to withstand antibiotics, the potential for antimicrobial resistance to cause a global health crisis looms large. Even though no statistically significant difference was observed, the number of Campylobacter species isolated from knives swabs was higher than personnel hands swabs. Research Highlights Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter. Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. You should upload this as a separate file labeled 'Manuscript'. Journal of Global Antimicrobial Resistance eISSN: 2213-7173 pISSN: 2213-7165 Open Access Share this on: Scope & Metrics Journal Specification Published Literature Similar Journals FAQs Key Metrics CiteScore 4 Eigenfactor 0.005 - 0.01 Impact Factor < 5 Scite Index 0.82 5-Year SI SJR Q3 Immunology SNIP 1.05 Topics Covered Staphylococcus aureus Genome However, Jimma Town is not as such small town and it is home for more than 207,573 (projected population in 2021/not actual) with 50.52km2 in south-western Ethiopia. The Fleming Fund (an aid programme supporting 24 countries across Africa and Asia to tackle AMR) was established in 2015. The journal encourages submissions from the research community where the priority will be on the originality and the practical impact of the reported findings. Totally, 684 samples were taken from apparently healthy bovine (faecal), their carcasses swab, personnel hand swab and knife swab, 171 samples for each sample type. In addition to their essential role in the clinic, antibiotics are used in a huge array of non-medical applications, from promoting growth in livestock, to preserving building materials from contamination, to treating blight in orchards. Costs of up to $29,000 per patient treated with an antibiotic-resistant bacterial infection have been reported. Sub-Saharan Africa and south Asia have the highest burden of AMR according to the new study, likely driven by high infection rates and a lack of access to antibiotics, compounded by serious data gaps. Isolated and identified species of C.jejuni, C. coli and C. lari accounted for 63.2%, 23.7% and 13.2%, respectively. Luber P, Brynestad S, Topsch D, Scherer K, Bartelt E. Quantification of Campylobacter Species Cross-Contamination during Handling of Contaminated Fresh Chicken Parts in Kitchens, Campylobacteriosis: the role of poultry meat. Permission was sought from Jimma Town Municipal Abattoir and informed consent was obtained from individuals providing slaughtering service to take personnel hand and knife swabs. Please remove any funding-related text from the manuscript and let us know how you would like to update your Funding Statement. CLSI guideline M45. In a PLOS Biology article, the authors sought to understand the properties that determine how quickly resistance will evolve [4]. Addressing the silent pandemic of antimicrobial resistance (AMR) is a focus of the 2021 G7 meeting. No, Is the Subject Area "Methicillin-resistant Staphylococcus aureus" applicable to this article? We thank the reviewer for the comments Line 15,18 and 23. We thank the reviewer for the comments and words mentioned in line 93 and 96 were replaced accordingly. As we have learned more about the mechanisms and epidemiology of resistance to antimicrobial drugs, it has become clear that bacteria have a remarkable array of tools at their disposal to. To obtain El Baaboua A, El Maadoudi M, Bouyahya A, Kounnoun A, Bougtaib H, Belmehdi O, et al. They help us to know which pages are the most and least popular and see how visitors move around the site. Federal government websites often end in .gov or .mil. Competing interests: LR is a current paid employee at Public Library of Science. Open Access, Research The 11 different antibiotic discs, with their concentrations given in parenthesis, used in the antibiogram testing were; Ampicillin (AMP) (10g), Nalidixic acid (NA) (30g), Tetracycline (TE) (30g), Ceftriaxone (CRO) (30g), Cefixime (CFM) (5g), Cefotaxime (CTX) (30g), Clindamycin (DA) (10g), Sulfamethoxazole trimethoprim (SXT) (25g), Ciprofloxacin (CIP) (10g), Chloramphenicol (C) (30g), and Cephalothin (CH) (30g). For more information about our data policy, please see http://journals.plos.org/plosone/s/data-availability. Please do not edit.]. There are many articles with similar objectives and I do not observe in this any original element beyond the region studied. Antibiotic resistance is a threat to the effective prevention and treatment of an ever-increasing range of infections caused by bacteria, parasites, viruses and fungi. In previous works, prevalence as well AMR was only determined from faecal but in our case it was from faecal, carcasses, personnel hands and knife samples. 2. Download PDF. 1. We are also thankful to technical staff of School of Veterinary Medicine for their assistance during laboratory works and Jimma University College of Agriculture and Veterinary Medicine. We thank the reviewer for the fruitful comments. School of Medical Laboratory Sciences, Institute of Health, Jimma University, Jimma, Ethiopia. Line 15 make the campylobacter italics Campylobacter species from bovine make. Most of the isolates were resistant to Cephalothin, Ampicillin and Cefotaxime. The journal welcomes manuscripts related to the epidemiology, surveillance, and prevention of healthcare infections and antimicrobial resistance. Wayne, PA: CLSI: 2015. Is the manuscript presented in an intelligible fashion and written in standard English? A major driver of AMR and poor clinical outcomesis suboptimal antimicrobial use. It exhibits resistance to a wide variety of antimicrobial agents and expresses diverse molecular epidemiology to various established classes of . official website and that any information you provide is encrypted . We thank the reviewer for the comment absence of molecular study. a. PLoS Biol 15(8): Personnel hands and utensils such as knife and cutting boards are among significant contributors for the contamination of animal food products with Campylobacter species [35]. A pair of synergistic antibiotics are more effective than the sum of the efficacies of each antibiotic when used alone and their dual action is thought to be more difficult overcome. In my mind the major limitation is the absence of genotypic studies for AMR and virulence of isolates it could have made your study more meaningful and interesting. We thank the reviewer comment on the tittle of the manuscript. b. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. The Institute of Medicine estimates the annual cost of infections caused by antibiotic-resistant bacteria to be U.S.$4 to $5 billion ().However, methods for measuring economic impact of resistance are in their infancy, and the studies leave many questions unanswered (). | Learn . They identify four mechanisms that have led to its new role in resistance, and most importantly, show that it was the use of antibiotics in medicine and in livestock feed that drove this evolution and spread of resistance. Antimicrobial resistance. Reviewer #3:accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte accepte. Although the emergence of resistant microorganisms is a natural phenomenon, selection is . To use PACE, you must first register as a user. They show that resistant bacteria can be re-sensitized by treating with a specifically designed anti-sense oligonucleotide. Hart Sam Kariuki View Show abstract Antibiotic resistance Article Sep 2016 Marianne Frieri Krishan. The publishing protocol for Journal of Global Antimicrobial Resistance is to publish new original articles that have been appropriately reviewed by competent academic peers. Personnel hand and knife swabs were collected after slaughtering each selected cattle. Global Epidemiology of Campylobacter Infection. Eight (8) disks were placed on a 150-mm plate (petri dishes) not closer than 24 mm (center to center) and the rest three on small 60-mm petri dishes on the Muller Hinton agar plate and incubated at 42C for 48 hours in anaerobic jar using microaerophilic condition generating sachet (CampyGen 2.5L Oxoid Ltd.). We included the importance of our finding in the revised manuscript. Antimicrobial resistance (AMR)the ability of a microorganism (bacteria, virus, fungi, parasite) to resist the effects of a drugis a serious, complex and costly public health problem. These cookies may also be used for advertising purposes by these third parties. 1. The magnitude of AMR is spreading more rapidly in the developing countries due to uncontrolled use of different antibiotics [10, 11]. We thank the reviewer comment on study design. The existing burden of AMR and its future threat should be an urgent stimulus to revise and widen The Global Fund's mission. The conclusions must be drawn appropriately based on the data presented. High Visibility: indexed within Scopus, SCIE (Web of Science), PubMed, PMC, Embase, CAPlus / SciFinder, and other . government site. The colonies of thermophilic Campylobacter from blood agar medium were picked up with a sterilized cotton swab and put into small tubes containing storage medium (Brain- heart- infusion broth medium) for identification. Polymyxins arrange the bacterial lipids into regular hexagonal structures that stiffen the membrane and lead to rupture. Objective to rephrase, not clear, and to show novelity or improvement from previous studies carried in this era. Review. Between 2014 and 2016, there were 700,000 deaths around the world attributed to antibiotic resistance. Jimma Town Municipal Abattoir gives slaughter services of cattle, sheep and goats. We included in the limitation of the study in the revised manuscript. However, funding information should not appear in the Acknowledgments section or other areas of your manuscript. As the name implies, these pumps expel drugs from the cytoplasm, limiting their ability to access their target. CDC. Yes The Fund was established to invest in programmes to end the infectious diseases that cause the greatest burden of illness and death, especially in low-income and middle-income countries. Importantly, these mutations promote further evolution of the bacteria in a multi-drug environment in a drug-specific manner, thus they both provide resistance and spur further development of resistance. Currently, your Funding Statement reads as follows: 4. Before from a pig slaughterhouse and analysis of cross-contamination. Preliminary identification of thermophilic Campylobacter species was performed based on microscopy to see characteristic darting motility by preparing wet smear or saline wet mount, with the iris diaphragm closed effectively to contrast the field. The study was conducted at Jimma Municipal Abattoir in Jimma Town. The growth of antibiotic resistance has stimulated interest in understanding the mechanisms by which antibiotic resistance genes (ARG) are mobilized. Internet Explorer). If you don't remember your password, you can reset it by entering your email address and clicking the Reset Password button. The inconsistency of the prevalence may be ascribed to geographical and environmental variability in the country. e. We thank the reviewer for the comment to use standard strain of Campylobacter. Soon after the discovery of the first antibiotic, the challenge of antibiotic resistance commenced. However, limited studies reported the occurrence and susceptibility testing of Campylobacter strains to antimicrobials on humans, food animals and foods of animal origin [1618]. The search must be at least three characters long. Background: In this first national bloodstream infection (BSI) surveillance program in China, we assessed the composition of pathogenic bacteria and the trends for antimicrobial susceptibility. 3. We thank the reviewer for the comments and paragraphs in line 38-39, 81-83 and 88-89 were reformulated in the revised manuscript. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". The concern around the increasing prevalence of drug resistant bacteria is compounded by the fact that the discovery of new antibiotics is a fleeting rare event. c) If any authors received a salary from any of your funders, please state which authors and which funders. On the other hand, the isolates were susceptible to Nalidixic acid (86.8%), Ciprofloxacin (86.8%), Sulphamethazole (84.2%), Ceftriaxone (78.9%), Clindamycin (68.4%) and Cefixime (65.8%). Registration is free. Is the Subject Area "Antibiotic resistance" applicable to this article? This is an open access article distributed under the terms of the, GUID:C7B84C0B-E296-4C15-869A-619DDB178CA5, GUID:CDFA03E4-BDA5-4857-B252-C5473DB4287A, GUID:0908447C-2678-4FC5-AE2E-C741E301D973, GUID:413F323D-7673-4097-B65C-19DDED51E239. The Pediatric Infectious Disease Journal: August 2021 - Volume 40 - Issue 8 - p 756-762. doi: 10.1097/INF.0000000000003134. Abdi-Hachesoo B, Khoshbakht R, Sharifiyazdi H, Tabatabaei M, Hosseinzadeh S, Asasi K. Tetracycline Resistance Genes in Campylobacter jejuni and C. coli Isolated From Poultry Carcasses. Hagos Y, Gugsa G, Awol N, Ahmed M, Tsegaye Y, Abebe N, et al. Our research finding determines the current status of Campylobacter species in Jimma Town Abattoir, source of contamination and level of antibiotic resistance. Antibiotic resistance data from starting up and experienced laboratories were presented, showing high rates of resistance in key pathogens to most antibiotics currently available in Cambodia, e.g .
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